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na 1 49 apo tirf  (Nikon)


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    Nikon na 1 49 apo tirf
    Na 1 49 Apo Tirf, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 57101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 57101 article reviews
    na 1 49 apo tirf - by Bioz Stars, 2026-07
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from <t>TIRF</t> to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.
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    (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from TIRF to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.

    Journal: bioRxiv

    Article Title: Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells

    doi: 10.64898/2026.03.23.710523

    Figure Lengend Snippet: (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from TIRF to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.

    Article Snippet: The Dragonfly was used in the BTIRF mode, applying an objective-type TIRF or widefield configuration with an oil-immersion objective (Nikon Instruments, Apo SR TIRF 60×, NA 1.49, Oil).

    Techniques: Imaging, Comparison, Binding Assay